|
KMID : 1123920090230051063
|
|
Korean Journal of Oriental Physiology and Pathology
2009 Volume.23 No. 5 p.1063 ~ p.1072
|
|
|
Effects of the Lonicerae Flos Extract on the Membranous Nephropathy Induced by Cationic Bovine Serum Albumin in Mice
|
|
Lee Joo-Ho
Cho Chung-Sik
Kim Chul-Jung
|
|
|
Abstract
|
|
|
|
Membranous nephropathy(MN) is the most common cause of adult nephrotic syndrome worldwide. But treatment of MN is not defined. This study was to evaluate the effects of Lonicerae Flos Extract(LFE) on the MN induced by cBSA in mice. Mice were divided into 4 groups. The first group named for ’Normal’ was injected with a saline solution. The second group named ‘Control’ treated with cBSA(10 ㎎/㎏ i.p) only. The third group named ‘LFE-250’, treated with cBSA(10 ㎎/㎏ i.p) and LFE(250 ㎎/㎏, p.o). The fourth group named ‘LFE-500’ treated with cBSA(10 ㎎/㎏ i.p) and LFE(500 ㎎/㎏, p.o). After cBSA and LFE treatment for 4 weeks, we measured change of body weight, 24hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN, creatinine, TNF-α, IL-6, IL-1β, IL-10, IFN-γ, IgA, IgM and IgG levels. The morphologic changes of renal glomeruli were also observed with a light microscope. The levels of 24 hrs proteinuria, total cholesterol, IgG, IgM, IgA, IL-6 were significantly decreased in both LFE groups. The level of triglyceride, IL-1β was significantly decreased in LFE-500 group. The level of Albumin was significantly increased in LFE-250 group. The level of TNF-α, IFN-γ were significantly decreased in LFE-250 group. The mRNA expression of IL-1β in splenocytes was consideraly decreased in LFE-500 group. In histological findings of kidney tissue, thickening of GBM decreased in both LFE groups. This study shows that the LFE might be effective for treatment of MN. More clinical data and studies are to be done for efficient application.
|
|
|
KEYWORD
|
|
|
lonicerae flos extract(LFE), membranous nephropathy(MN), cationic bovine serum albumin(cBSA)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|